NYK and Astomos Energy Corporation have participated in a marine biofuel demonstration project launched by the Global Center for Maritime Decarbonisation, a non-profit organization aiming to promote decarbonization of the maritime industry.Biofuels can be used without remodeling the existing ship’s engines and infrastructure. Furthermore, although biofuels generate CO2 when combusted, they are considered carbon-neutral because they are made from plants and waste cooking oil. So, biofuels are expected to be one of the most promising next-generation fuels for decarbonization. On the other hand, since biofuels have not been put into practical use in earnest, many data verifications, such as the mixing ratio with conventional fuels, are still needed.In addition, since biofuels use fossil fuels in their production and transportation processes, they are not considered net-zero emissions in LCA*. Moreover, the certification scheme is not standardized internationally, so the transparency of the biofuel supply chain is also an issue.